Sentence examples for highest transformation efficiency from inspiring English sources

In general, electroporation offers the highest transformation efficiency, and is especially useful when small quantities of DNA are transformed.

To the best of our knowledge, this is the highest transformation efficiency that has been reported for Dietzia bacteria to date.

Testing of different T-DNA element vectors engineered for transformation of fungi showed that pBHt2 provides the highest transformation efficiency and the lowest rate of vector backbone carryover.

Overall, optimum values of 30 kDa and 1 may be suggested for the molecular weight and N/P to obtain size of 125 nm for pDNA/chitosan nanoparticles, with highest transformation efficiency (Fig. 3).

Effect of chitosan molecular weight, N/P ratio and NPs size on transformation efficiency was investigated and highest transformation efficiency was obtained at 30 kDa, 1 and 125 nm for chitosan molecular weight, N/P ratio and NPs size, respectively.

The highest transformation efficiency of butanol-tolerant GRSW2-B1 at 5.17 × 103 CFU (μg DNA -1 was achieveDNA -1 the competent cells wase prepachievedm cells growhenn LB medium thelate-exponential phase with OD600 of 0.6, and washed with icompetentSMG buffer.

The latter approach yields higher transformation efficiency (4~5 transformants per μg of DNA), but the former has the potential to mass-produce competent cells for future needs.

In conclusion, with the optimized approach, a high transformation efficiency of 145 transformants per 10 conidia was obtained.

The gene gun transformation showed higher transformation efficiency than the ones transformed using Agrobacterium when targets were bombarded once or twice using 280 psi of helium pressure at 6 to 8 cm distance.

Both genes were successfully transformed into Y. lipolytica Po1g with a high transformation efficiency, about 3,000/μg DNA.> -wrap-foot> The strain used for the enzyme expression was Y. lipolytica Po1g (MATa ,leu2-270 ,leu2-270 ,ura3-302 ,ura3-302 URA3 ,xpr2-332 ,xpr2-33227].

In addition, yeasts are attractive hosts for the high throughput screening of peptides or proteins from large combinatorial libraries (Chao et al. 2006; Li et al. 2007; Ueda 2004), with a high transformation efficiency of plasmids of up to 1 1.5 × 108 transformants/μg achieved (Benatuil et al. 2010).