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To the best of our knowledge, this represented the highest titer reported to date.
This strain produced 39.93 g/L of 5-AVA, which is the highest titer reported to date in fed-batch fermentation from glucose.
The TAG levels achieved here are the highest titer reported in S. cerevisiae, reaching 27.4% of the maximum theoretical yield in minimal medium with 2% glucose.
Finally, fed-batch fermentation in a 5-L bioreactor led to production of ~300 mg/L Rh2, which was the highest titer reported.
Further overexpression of glycolate oxidase and catalase significantly improved glyoxylate production to 0.74 g/L, which is the highest titer reported to date.
The engineered strain is able to produce 1.2 g/L of SA from simple carbon sources, which is the highest titer reported so far.
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Interestingly, replacement of ammonium salts with urea resulted in production of 54 g/L ethanol, one of the highest titers reported for Thermoanaerobacterium.
For the purpose of calculations, negative titers reported as <10 were assigned a value of 5, while high titers reported as greater than or equal to a given value were assigned a value corresponding to the next 2-fold titer; e.g., the value of ≥1,280 was assigned a value of 2,560.
So far, in literature the highest rhamnolipid titer reported using heterologeous production hosts was 7.3 g/L (Cha et al. 2008).
Highest MAT titer reported to any leptospira serogroup.
To the best of our knowledge, these results constitute the highest adipic acid titer reported in E. coli.
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