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For the highest stringency of 2-fold, we observed 492, 357, and 325 genes for profile-1, -2 and -3 respectively.
Analysis of the 2000-bp upstreaMrc1c1 promoter for GRE sequences across species was conducted using the ConTra online software (Hooghe et al, 2008), with the highest stringency of core = 1.00 and similarity matrix = 0.95 and a method to minimize false positives with TRANSFAC matrices.
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Consistent with the predicted high stringency of SLiMFinder, two of the datasets yielded no significant results.
Compared with ELISA assay, pull-down assays require higher affinity between the ligand and receptor due to the high stringency of particle precipitation and washing steps.
Clustering of ESTs performed by the EST2uni-pipeline [30] resulted in a slightly smaller number of ESTs passing quality due to the higher stringency of the set parameters (Table 4).
The high stringency of the statistical treatment according to Bonferroni criteria (Bonferroni p-values≤0.05) was confirmed for genes that were barely detected, such as PARP or DNA polymerase ε, or not detected (ku70, lig4, brca2) by microarrays.
Due to the high stringency of our search, ESTs for genes traditionally known as melanocyte/melanoma-specific genes, such as TYR (Hs.503555), DCT (Hs.301865), MLANA (Hs.154069), and SILV (Hs.95972), were not selected because they also match entries of other tissue datasets.
For the LCR samples (Fig. 2B and D), increased target yield and specificity of both IGF2R and BRAF genes compared with PCA (Fig. 1B and D), is due to the higher stringency of the ligation method; only those overlapping fragments that are perfectly matched are ligated together [35].
No probesets met the high stringency of a false-discovery correction for multiple comparisons.
Assembly 2 is an initial assembly at a higher stringency of 1×10-60 with 17,832 contigs.
This might be due to the higher stringency of the system to minimise nonspecific WBC signals.
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