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Their interaction partners outside the HLA region where included at the highest stringency level to visualise informative interaction pathways in which the HLA proteins are involved.
We have shown that an edge-swapping procedure used to randomize the bipartite graph generates a null-model that allows co-occurrence analysis with the highest stringency.
After 36 48 hours of incubation, colonies were harvested from the highest stringency plate yielding at least 1000 colonies as previously described [26].
As outlined above, it is expected that the null-model that yields the highest estimates of expected co-occurrences should permit co-occurrence analysis with the highest stringency.
We conclude that the ES null-model is the null-model that permits co-occurrence analysis with the highest stringency among the models tested and that it closely approximates occurrence probabilities derived from an ideal permutation model.
As is evident from figure 6A, the interaction of MP with VPg was abolished at the highest stringency upon deletion of the N terminal 49 and C terminal 19 amino acids of MP.
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Washing was conducted in PBS/tween-20 with one high stringency wash at 57°C.
The membrane was subjected to a series of stringency washes (high stringency at 56°C) to eliminate nonspecific probe binding.
A high stringency protocol, suitable for systematic purification of polyclonal antibodies, is described.
All of the 22 tested T0 transgenic lines showed HptII banding after the high stringency hygromycin selection (Fig. 2b).
Solution 3 (high stringency) contained 0.1× SSC.
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