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In order to obtain the most informative sequences, the amplicon with the highest size for each DRT primer mix in each applied DNA walking method was selected to be sequenced (Additional file 5).
Under these conditions and without misclassified causes of death, the results in Figure 4 (see also Additional file 1: Figure A.6 and Table A.5) suggest that Gr has the lowest power for CD (0.54 < 1- β < 0.93 for Gr, while 0.86 < 1 - β for Pe and CS; complete data) and often by far the highest size for NCD (0.16 < α; complete data).
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In that case, Pe was comparable to CS, except that CS had very high size for NCD in the presence of a high rate of misrecorded causes of death.
After shore surveys, shipboard surveys had the next highest group size for marine mammals (mean = 16) though only slightly higher than data gathered from aerial surveys (mean = 14).
Paralysis, faints/blackout, breathlessness, and pain were the impairments with the highest effect size for less wellbeing.
For each, we gathered allele frequencies for Europeans and East Asians from either public databases (HapMap, ALFRED or dbSNP) or, if not available, from the paper with the highest sample size for each Continental population.
A higher effect size for alcohol outcome was associated significantly with a higher effect size for depression (β = 0.511, 95% CI = −0.04 to 0.99, P = 0.003); the reverse relationship was not significant.
In our study, however, a higher effect size for depression outcome did not correspond significantly with a higher effect size for alcohol outcome, as was the case in Nunes & Levin's meta-analysis of antidepressant medication.
Excess eggs (e.g. a fourth egg on a four-egg clutch) were assigned to the last group, which resulted in a somewhat higher sample size for this group.
These studies demonstrated a high effect size for outcome measures (d = 1.2 - 2.4).
Heat effect was also found for 15-day average temperature and respiratory mortality, but with slightly higher effect size for elderly people.
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