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The clone which was used for further experiments secreted 2 ng PEDF/10 cells/day, as analyzed by ELISA and immunoblotting); the highest secretion level was reached 1 week after transfection and remained constant throughout the 10 passages the cells have been followed in culture.
TNFRI-Fc induced the highest secretion of IL-10 and MCP-1.
The colony with the highest secretion level of NX1α III -MycHis was cultured iNX1α III -MycHis (Corning Glasswasks, cultured NY).
Although similar results were seen with MOIs from 6 to 100 for all four chemokines, the highest secretion of CCL4 occurred with an MOI of 50.
Various carbon sources were tested; among the tested Broths C/E/F/H/I, Broth H achieved the highest secretion productivity of rTFF1 (170 mg/L) at 48 hr.
Broth H with higher glucose content (50 g/L) achieved the highest secretion productivity of rTFF1 (170 mg/L) at 48 hr of culturing; enhanced productivity and less culture time to achieve optimal production were both achieved.
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Compared with the poly l-lysine)–VEGF compoly l-lysinesome–VEGF revealed a high secomplex of VEGF and low toxicity.
Semi-scale protein production and purification using HiTrap-PA column showed a high secretion of the recombinant protein by Western blotting method.
The HSEs with neurons and hypodermis, compared to in vitro skin-only HSEs controls, demonstrated higher secretion of pro-inflammatory cytokines.
Results: CD62LLow T cells demonstrated significantly higher secretion of interleukin (IL -10 and IL -10eron (IFN)-γ thanddinterferongh T cells.
The histological and immunohistochemical results confirmed that the primary chondrocytes cultured into the chitosan-gelatin scaffold maintained round and owned characters of high secretion of specific ECM.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com