Exact(3)
COI and 18S were the highest quality sequences (0.91 and 0.88 respectively), WG was intermediate (0.78) between these and EF1a (0.61) and CAD (0.53) sequences (Figure 1B).
Cluster representatives were chosen based on phrap quality scores to use the highest quality sequences for further analysis; for large clusters, multiple representatives were chosen.
The 2,678 highest quality sequences over 100 bp in length are reported as FF682838 through FF685515 in GenBank.
Similar(57)
An average of 35595 high quality sequences per sample was obtained from 12 colonic content samples (Supplementary Table S1).
The qualitative and quantitative analyses performed on the ready-to-sequence libraries were a crucial step for achieving high quality sequences.
In order to obtain high quality sequences, tags with length of <220 nt, average quality score of <20, and tags containing >3 ambiguous bases were removed by PANDAseq.
These high quality sequences had an average read of 500 bps.
All the high quality sequences were classified into 99 OTUs (97% similarity cutoff).
The number of total high quality sequences obtained from raw pyrosequencing data was 4586 reads.
A total of 211 clones were picked, and 190 high quality sequences were obtained.
More than 112,000 high quality sequences from isolates and uncultured strains were downloaded as FASTA files.
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