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This ensured only the highest quality reads were used for subsequent de novo assembly.
The highest quality reads are the ones on the right hand side of the plotted band in Figure 2 and, clearly, still contain an unacceptable proliferation of technical variants.
This is important as Dohm et al. [ 64] found that the error rate provided by the Solexa platform may underestimate the true error by a factor of up to 100 for the highest quality reads.
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As additional quality criteria we used only variants with a coverage >10× of high quality reads.
The remaining high quality reads were checked for the existence of chimeric sequences.
The GS20 has standard software to recognize high quality reads and convert the signal (light) into a base call.
They were regarded as high quality reads.
This resulted in 1,517,878 high quality reads.
Thus filtered, high quality reads were used for further analysis.
For this purpose, all 1,063,520 high quality reads were used.
Only high quality reads were provided by BGI.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com