The TAP tag was more recently developed in order to obtain high purified protein samples for mass spectrometry analysis of protein complexes [ 29].
Thus, gene duplicates in unicellular organisms are subject to higher purifying selection than their counterparts in multicellular eukaryotes.
These results suggested that further technical improvements were necessary to achieve high quality purified proteins with high-efficiency from the cell-free based His-srtA system.
The oligonucleotides were of highest quality, purified either using HPLC or from PAGE gels.
The protein has been expressed to a high level, purified from Escherichia coli cells with a high yield and its catalytic properties compared to popTrxh1.
He stresses, though, that experiments use high concentrations purified from raw material.
The apparent K m of immobilized laccase nanoparticles was found to be 0.5 mM which was 21.05% higher than purified laccase (0.19 mM).
Limited information or methods are available for producing high-titer purified phage suspensions for studying microbial transport and survival in natural and engineered environments.
The availability of large amounts of high stability purified recombinant proteins could facilitate further studies on its biological activities.
Expression levels of cyclin E and cyclin D1 were markedly higher in purified HEY PGCCs than those in the control HEY cells.
