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The highest protease production by A. oryzae was achieved at a fixed air flow rate of 12 mL/min, with inlet air relative humidity within the range 66 94% and initial substrate moisture content between 30% and 40%.
The highest protease production was found using FermII media.
The highest protease production was observed in glucose and casein, which is in conformity with an earlier study [ 12].
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Soy fibre presented the highest yield for protease production at the laboratory scale (37 °C, 100 g samples).
Given its high level of protease production by comparison to clinical isolates, one possible explanation for this is that the increased production of extracellular proteases also limits nuclease activity in RN6390 irrespective of the functional status of sarA.
Twenty-four dog isolates (24/44, 54.5%) and none of the pigeon isolates were positive for clumping factor and protein A. Protease production was significantly higher in dog isolates (24/44, 54.5%) than pigeon isolates (11/62, 17.7%) (p < 0.0005, Fisher's exact test).
Based on a previous study carried out in our laboratory, it is known that plasmid segregation occurs in the same pET expression system for ClpP protease production, even at the highest kanamycin concentration evaluated in this study [ 31].
Compared to the Erlenmeyer flask, the CCF allowed protease production that was 30% and 35% higher and growth that was 20% and 345% higher for DGII and V. pantothenticus, respectively.
The results confirmed the high biotechnological potential of this fungal strain for neutral protease production in solid-state fermentation.
Based on protease production, the strains were classified as high, medium and low protease producers.
Further substantiating this, protease production, known to be activated by high levels of DegU∼P, was not stimulated by the degS S76D mutant (data not shown).
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