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The highest production yield of pectin (21.85 ± 0.35%) was obtained at temperature of 90 °C, extraction time of 180 min and LSR of 40 v/w as optimum extraction conditions which was close to the predicted value (24.13%).
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In a simple iterative knockout screening method, the effects of all possible single reaction knockouts on metabolic fluxes are evaluated in the first iteration, and the reactions whose knockouts result in the highest score (e.g. highest production yields under biomass production maximization) are selected.
Transient expression in HEK293-6E cells using these optimized pCSE vectors containing the scFv-Fc and scFv-Fc-RNase expression cassettes resulted in similar or better yields than the corresponding pCMV-oriP1 test vectors which achieved the highest production yields compared to all previous vectors.
Various sets of knockouts resulting in positive or zero values were collected, of which 30 unique reaction knockouts that contributed to the highest production yields were used as knockout candidates for the OptKnock screening with several maximum numbers of knockouts (3, 5 and 10 KO).
This synthesis method is simple with very high production yield and does not require any post-treatment procedure, and products can be collected from organic phase which effectively avoids TiO2 grain aggregation.
A 2.7× higher production yield was achieved with EV71 compared to CVA16.
The fundamental purpose of a fed-batch process is to achieve a high production yield at the low cost.
Here, it is pertinent to mention that CD alone can act as an effective catalyst with higher production yield (Fig. S4).
Furthermore, the radiosynthesis of [11C]CB184 was straightforward with high production yield [19] that will meet the GMP standards for human use.
Phenolic resins are usually selected as carbon precursor because it gives high production yield and allow the production of porous carbon with high surface areas and tailored porosity.
CVD is the most popular and extensively used method because it is easy to scale-up, can be performed at a lower temperature [6], has a lower set-up cost and higher production yield than other approaches [1].
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