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Clearly, sorghum DArTs meet this criterion, with over 69% of the 508 DArTs revealing polymorphism between the set of 90 sorghum lines having a PIC value between 0.5-0.4; 0.5 being the highest PIC value expected for a bi-allelic marker system.
A primer (UBC827) showed the highest PIC value (0.98), thus detecting to the highest level of polymorphism.
The highest PIC and GD values were equivalent to those observed in tropical and temperate lines [ 12, 31].
Marker CYP21 showed the highest PIC value (0.87) and marker BMC1013 showed the lowest PIC value (0.24) (Additional File 1).
The highest PIC value (0.34) was observed for the primer combination MSeI-CAA/ EcoRI-ACT and the lowest PIC value (0.26) was recorded for the primer combination MSeI-CAC/ EcoRI-ACA (Table 2).
In terms of diverse kinds of SSR motif, using the 'Di' type of SSR as an example, GA & CT motif-containing markers gave the highest PIC value, while the CG & GC motif-containing markers showed the lowest genetic diversity among the accessions sampled in this study (Additional file 4: Table S2, Additional file 2: Figure S1).
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We also trimmed the TM data to the 415 loci with the highest PICs to compare the most informative TM loci to the AHE loci.
The mean polymorphism information content (PIC) for all the loci was high (PIC > 0.7), suggesting that these microsatellite markers could be useful for population genetics and diversity studies.
In agreement with prior data about condition-specific genes [ 8, 25], genes with high EV mainly have "covered" promoters with reduced histone acetylation and no RNA polymerase pre-initiation complex (PIC) occupancy, while genes with low EV have high PIC occupancy and increased histone acetylation in their promoters.
The 160-acre Loterie Farm, once a sugar estate and now a private nature reserve, encompasses the island's highest point, 1,400-foot-high Pic Paradis.
By contrast, SSRs isolated from sugarcane genomic libraries amplify more readily, show high levels of polymorphism within sugarcane with a higher PIC value (0.72) but do not transfer to related species or genera well.
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