Careful attention must be paid to drafting the search request in a way that corrects for the high number of variations in spelling and typographic errors in the database.
On the other hand, the genome from an organism with repeat regions or highly variable sequences may show a much higher number of variations compared to its most closely related reference genome and hence prove difficult to assemble and take longer than 12 hours for downstream bioinformatic analyses.
Use of a 3'UTR directed approach exploits the higher number of variations found in the 3'UTR region compared to the coding region of a gene.
Because of the high number of variations, the gene identity with D. solani strain 3337 decreased in RGT49134, especially in the norR gene that was located in the central part of the RGT region.
All APO-UNSUS rats tested (n = 5) indeed showed the database sequence, whereas the APO-SUS rats tested (n = 5) all contained the relatively high number of variations in this chromosomal region.
The proportion of mutations causally implicated in cancer is still unknown especially due to the high number of variations between different tumors (5 9) Although the number of unique variations for each cancer genome can be very high (10, 11), only a few somatic variations will be critical for the development of the tumor.
R08-1450 and Jack, which contained the highest number of genes exhibiting significant expression variation, had 3,357 and 1,478 genes, respectively (Additional file 1: Figure S2).
Using pan-genome analyses, we identified more than 10,000 novel full-length protein-coding genes and a high number of presence absence variations.
A higher number of metabolite variations were detected in leaves compared to roots during short-term response to N availability.
However, especially if large sets of positional candidates are involved, a high number of sequence variations can be expected to be found.
