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Nigrospora sp. showed the highest gene expression of Lcc under the same conditions of Lcc synthesis.
Among all the oligomerized peptides, tri-RC29 provided the highest gene expression efficiency, and its peak luciferase level was 1.5 times higher than that yielded by PEI at its optimal N/P ratio (i.e. 10).
Whereas gene expression of the phenotypic markers measured using real-time reverse transcription polymerase chain reaction (RT-PCR) indicates ACL cells have the highest gene expression for the matrix markers.
Analysis on single pattern structures showed that 2 μm gratings with 2 μm spacing and 2 μm height (2 μm gratings) and 2 μm gratings with hierarchical structure produced cells with the highest gene expression of TH and PITX3, with the longest neurite and highest percentage of alignment.
The relative gene expression of each tumor was computed by normalizing its value to the tumor with the highest value (a value of 1 corresponds to the tumor with the highest gene expression).
We would like to note however, that all these positive conclusions about performance of SAM and Limma methods were obtained with use of 2-step normalization procedure, which demonstrated better performance in the most important area of highest gene expression (Figure 4 and Supplemental Figure S1).
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In most GIRK1 high gene expression cases, AMF-R gene was also expressed.
The ubiquitously expressed housekeeping genes are also seen to execute high gene expression level [ 14].
But "the authors suggest that all they need to do is use more cells and get higher gene expression," Dr. Miller added.
Also, a higher gene expression of Sox9 was found.
The results of transfection study in vitro indicated that LPCM exhibited higher gene expression than PIC.
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