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Both the control and the gpd1Δ gpd2Δ strains showed the highest fluorescence at the beginning of the cultivation, followed by a gradual decrease until the end of fermentation.
The pH dependent fluorescence spectra (pH = 1 9) showed that all the complexes exhibited pH-dependent fluorescence in vitro, with highest fluorescence at acidic pH (Fig. S5 and S6 †).
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Here, the remarkable decrease of fluorescence intensity can be explained as follow: The fluorescence intensity of BHN-Fe3O4@SiO2 BHN-Fe3O4@SiO2ted at a 415 nm lamp, exhibits the high fluorescence at 518 nm due to the 1,8-naphthalimide which has a bis conjugatexcitedem.
When measured 2 6 h after treatment, the magnitude of DCF fluorescence increased in both −80 and −46 mV conditions, with at least a 2-fold higher fluorescence at −46 mV (P<0.001, data not shown).
Moreover, cells infected with bacVP6 had a high fluorescence at 575 nm.
The results are expressed as the percentage of positive cells showing high fluorescence at FL3.
Serum ACE2 activity could not be measured in one control patient and two healthy subjects due to high fluorescence at the start of incubation, and sera from the remaining seven control patients were not available for these experiments.
The reaction product was found to be fluorescent showing the highest fluorescence intensity at λex of 382 nm and λem of 495 nm.
These cells were manually tracked in Omero by identifying the Z plane presenting the highest fluorescence intensity at each time point.
In case of 25 μM H2O2, we observed the highest fluorescence intensity (at 2 min), which decreased with increasing H2O2 concentrations.
Furthermore, the highest fluorescence occurred at an intracranial depth (5 6 mm) that corresponded closely with the intracerebral stereotactic implantation of U87 cells.
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