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Algae grown under medium nitrogen concentration (10 mM) and low phosphate concentration (20 μM) had the highest FAME productivity (426.0 mg/L ± 135.0 mg/L).
The highest FAME yield of 92.67 ± 3.23% was obtained at 215 °C, 6.5 MPa and methanol to lipid mass ratio of 5 1.
Subsequently, a highest FAME productivity of 265 mg L−1 day−1 was observed in last two days of lipid induction phase.
The highest FAME yield of 99.0% was obtained over 3 wt.% of Ca OCH3 2 catalyst loading at methanol to oil molar ratio of 30:1 and reaction time of 3 h at 80 °C.
The FAME yield from algae grown under low nitrogen (3 mM) and phosphate concentration (20 μM) increased throughout the experiment and the highest FAME yield (42.2 ± 2.5% of AFDW) as well as C16 and C18 content (95.8 ± 1.6% of AFDW) was achieved under these conditions.
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Consistently high FAME yields, in excess of 80%, were obtained when the immobilized lipase was reused in six replicate trials at 10 and 20 °C.
In this sense, oils with higher unsaturation degree and long hydrocarbon chains provide higher FAME yields and lower glyceride concentrations than those with saturated fatty acids and short hydrocarbon chains.
A high FAME content (98.5%) could be achieved at the two thirds of the bed of quicklime bits without loss of catalytic activity within 30 h of continuous operation.
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The biomass grown on WCO had a lipid productivity of 0.042 g L-1 h−1 and 4 g of this loading gave a high FAME yield of 0.88 g in 8 h at 50 °C with methanol: chloroform (10 1) and acid catalyst (0.2 M H2SO4,1.0 ml g−1).
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