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During ZE maturation the expression of 6 of the 9 differentially expressed LEA genes increased with development reaching their highest expression values at the final M-ZE stage; the remaining 3 LEA genes demonstrated the opposite pattern with reduced M-ZE expression.
The description of brain GAPDH mRNA mean expression values of different groups at different time intervals showed that the highest expression values were represented at the 0-time (control group) and the lowest values at 96 h with an exception in group 4 that showed higher expression level at 24 h relative to the control.
Caspase-8 showed the highest expression values, reaching a 75-fold change maximum value over the control after 6 h pt.
In general, the highest expression values for all caspase genes were detected in glands, ranging from 95 times greater than muscle for caspase-3/7-2 to 106 times for caspase-2.
For each of the genes discussed where more than one probeset referred to the same gene annotation, only the best performing probeset with the highest expression values was used in order to avoid bias towards transcripts with more than one probeset.
The highest expression values were reported on day-35 with decreasing relative expression thereafter.
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Of these, MiR-126-3p has the highest expression value with ΔCT value 0.14 (i.e. copy number 159299).
Cyan indicates the lowest expression value, and pink indicates the highest expression value.
A major isoform is defined as the transcript with the highest expression value within a given sample.
For alternatively spliced transcripts encoding the same protein, only the transcript with the highest expression value was used.
In nervous tissues, Type 3 showed the highest expression value followed by Type 2, Type 5, and Type 1.
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