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Of these, MiR-126-3p has the highest expression value with ΔCT value 0.14 (i.e. copy number 159299).
Nevertheless, patient 9 did not influence the global gene expression analysis, while for the qPCr analysis (using a separate RNA/Analysis technology) patient 9 did demonstrate the highest expression value for NRF2 (See results) indicating that this patient's gene expression status was genuinely altered to a much greater extent.
The τ of miRNA i is defined as <img src="http://journals.plos.org/plosone/article/asset?id=info?doi/10.1371/journal.pone.0003420.e001.PNG" class= inline-graphic"/> where nH is the number of human tissues (here nH = inline-graphicax) is the highest expression value of miRNA i across the nH tissues.
Cyan indicates the lowest expression value, and pink indicates the highest expression value.
A major isoform is defined as the transcript with the highest expression value within a given sample.
For alternatively spliced transcripts encoding the same protein, only the transcript with the highest expression value was used.
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Caspase-8 showed the highest expression values, reaching a 75-fold change maximum value over the control after 6 h pt.
In general, the highest expression values for all caspase genes were detected in glands, ranging from 95 times greater than muscle for caspase-3/7-2 to 106 times for caspase-2.
The highest expression values were reported on day-35 with decreasing relative expression thereafter.
These highest expression values in healthy donors were used as "cutoff" to determine CTCs positivity.
In PC15, the highest expression values corresponded to capC and capA2 (30.4 and 22.1 RQs).
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