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Therefore, efforts were made to achieve a formulation with the ideal size of about 100 nm and below and the highest encapsulation efficiency.
The microspheres fabricated at 4 and 38°C yield the highest encapsulation efficiency (52.0 48.0%) and lowest initial BSA release (18.8 20.0%), while microspheres produced at 22°C have the lowest encapsulation efficiency and highest initial burst.
Heat-denaturation of the protein prior to microgel formation led to the highest encapsulation efficiency and retention, which was attributed to the formation of a cold-set protein gel inside the beads.
The different results obtained for the two drugs indicated the importance of the greater or lesser drug lipophilicity in determining the optimal bead composition with the highest encapsulation efficiency.
Optimization demonstrated that only minimal range of design space could be used to achieve the highest encapsulation efficiency (EE, more precisely the EE of 90% was gained using 25 mg/ml of lipid and drug loading of 0.3% (w/w).
The conditions gave the highest encapsulation efficiency (85.79% ± 1.65%) with the low value of the particle size (180 nm ± 4 nm), and the experimental values were close to the predicted values (Table 4), which indicated that the optimized preparation conditions were very reliable.
Similar(52)
Our results demonstrate that by this method we can generate homogeneous complexes (ca. 400 nm diameter, PDI 0.29 ± 0.07) with a very high encapsulation efficiency (about 99% encapsulated FGF-2).
GA nanoparticles could encapsulate cordycepin at higher encapsulation efficiency due to the attractive electrostatic interaction between the positive-charged GA and the negative-charged cordycepin.
GA nanoparticles could encapsulate cordycepin at higher encapsulation efficiency due to the attractive electrostatic interaction, however, GA nanoparticles released higher amount of cordycepin because of the large surface area of small size nanoparticles.
The addition of PEI appears to increase enzyme incorporation producing a biocatalyst with high encapsulation efficiency in terms of percentage of encapsulated protein (90%) and activity (47%).
siRNA-encapsulating PLGA microspheres were characterized by a high encapsulation efficiency and a slow and prolonged anti-TNF-α siRNAs.
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