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In contrast, the highest editing frequency (6.9%) occurred in petD, where the protein identity increased only 3%.
We found some 30 microRNAs that pass the filter (Table 2), with mir-986 and mir-971 being amongst the microRNAs with the highest editing level.
The authors also tested various lengths of ssODNs (30 110 nt) and their orientations with respect to the gRNA, finding that 70 nucleotide-long oligonucleotide templates complementary to gRNA enabled the highest editing efficiency.
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Fig. 1 Seven newly identified edits with high editing efficiency in protein-coding transcripts of moth orchid.
Two edits, petL-56 and rps12-221 are in high editing level (>83%) both in leaf and floral tissues.
We designed the KamiCas9 self-inactivating editing system to achieve transient expression of the Cas9 protein and high editing efficiency.
In contrast, petG-56 and rps3-583 are in high editing level (64 72%) in flower, but relatively low (30 35%) in leaf.
Additionally, we found higher editing efficiency for HF2-BE2 than BE2 in mouse embryos, when guided by full-length gRNA-2.
Seven edits in six protein coding transcripts having relatively high editing efficiency (>40%) were previous unidentified in moth orchid (Additional file 1: Table S2).
Of the two base editors (BE), BE2 (rAPOBEC1-dCas9-UGI) and BE3 (rAPOBEC1-nCas9-UGI), BE3 showed higher editing efficiency (Kim et al., 2017a).
The CRISPR system has recently emerged as a powerful gene manipulation technique with advantages of high editing efficiency and low cost.
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