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We demonstrate that the protein with the highest affinity for a particular DNA site need not be the protein that binds that site with the highest degree of specificity.
Analysis of the AA sequence of human HDx-1 using the web application SitePrediction identifies AAs 12-17 as having the highest degree of specificity for both calpain 1 and calpain 2, with a secondary recognition site at AA 5-10.
Ifenprodil and PEAQX concentrations were chosen according to previous reports demonstrating highest degree of specificity.
Although the FMIA showed an identical sensitivity as the bELISA, it demonstrated the highest degree of specificity of all three assays at 99.2 %.
Concentrations of inhibitors were chosen according to previous reports demonstrating highest degree of specificity and/or therapeutic relevance (APV and MK-801; memantine).
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The combination of gene specific primers and a gene specific probe ensures a high degree of specificity.
A high degree of specificity among human SH2 domains, combined with cell-specific expression is consistent with the notion that evolutionary pressures drive selectivity of protein-ligand interactions.
Because such hydrolytic analytical methods are not specific to diisocyanates, other methods with a high degree of specificity have been developed.
Colby and Chamberlin (1969) presciently concluded that the high degree of specificity of the induction process was consistent with the existence of a specific intracellular receptor, most probably a protein.
According to relevant literature, each primer set allows specific detection of the respective potato pathogen(s) with high degree of specificity and reliability.
Our data are consistent with this hypothesis and further pinpoint which specific step of the infection process is responsible for the high degree of specificity.
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