On the one hand, the C. rubella genome is by far the highest-quality reference genome, and in most cases we detect the highest copy numbers using this genome as the reference (compare Figure 3 with Additional file 2: Figure S2).
Expression was comparable between the BAC-Ctl3 mice and the BAC-Exp1 line, which has the highest copy number (13 copies).
The dots are diffraction limited and even in the case of the highest copy number gene β-actin, the dots were non-overlapping.
Expression in BAC-Ctl3 mice was significantly higher than in the BAC-Exp2 line, which has the second highest copy number (9 copies).
We detected 19 cellulose synthase (CesA) and 38 cellulose synthase like (Csl) genes24,25 in the bamboo genome, representing nearly the highest copy number of these genes among the 7 sequenced plant genomes (Supplementary Table 14a).
Expansion lines with the highest copy number, BAC-Exp1 and BAC-Exp2, develop a severe, fully penetrant phenotype (see Supplementary Video 1 online, which shows a BAC-Exp2 animal at 20 weeks) in which animals become inactive, lose weight and die prematurely, with survival ranging from 4 18 months (Fig. 2a and Table 1).
Among the mutants, ∆fob1Sup13 and ∆fob1Sup16 showed the highest copy number (ca. 800 copies).
In the teteraploids, Koala (O. coarctata), Hopi (O. ridleyi), Dasheng (O. minuta), Atlantys (O. alta and O. minuta), and Houba (all tetraploids) were the highest copy number elements.
The total miRNAs copy number of 8052, miR-191 had a highest copy numbers (1088) and miR-25 had 120 copy numbers, among the eight miRNAs picked up in this assay.
The sequence with the highest copy number was considered the putative novel mature miRNA.
The highest copy number segment harbored 12 genes, including GPC5, GPC6 and ABCC4.
