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A notable difference is nevertheless observed at the highest conservation level (above 90% identity), where vertebrates sequences greatly outnumber those from insects (1A, ~4 times more conserved information in the highest identity class).
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RGA primer sets, designed on 17 reference RGAs, amplified additional RGA sequences from two hop genotypes which served for the development of 11 RGA markers of which eight amplified PCR products of expected sizes also in various hop cultivars, indicating a high conservation level of RGA sequences within the hop genome and two RGA markers segregated in the mapping family.
Such sampling had undoubtedly achieved very high conservation level of "alternative exons".
In this paper, we found that constitutive exons have higher conservation level in exon regions and lower conservation level in flanking intron regions compared to conditional exons.
This sequence showed a high conservation level of 65% identity when compared to other Gnmts from mouse, human, fish and pig (Figure 2B).
Although the predicted cancer driver mutations did fall at the positions exhibiting slightly higher conservation level, as compared to the passenger mutations, the difference was rather modest.
The high conservation level in the amino acid sequence of GK1 is well reflected in the models shown in Panel C of Figure 2. The two variants in the sequence of GK1, group the organisms in T. solium, T. saginata, and E. granulosus and T. crassiceps ORF and WFU, and E. multilocularis are shown.
Higher phyloP score means higher conservation level.
Lower weighted entropy denotes higher conservation level while higher structural sensitivity indicates more severe structural constraints.
The predicted translations of four unigenes exhibited a high conservation level (from 57 to 85% identity, table 4) with lepidopteran PRs previously described [ 21, 22, 26].
In this case, gene sequences were extracted manually and unambiguously, given the high conservation level of start and end positions shared by all 30 other annotated genes.
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