Sentence examples for highest confluence from inspiring English sources

An effect of age of the donor (6 months, 3,5,14 and 15 years) was not shown, but for all horses, in the monolayers of the highest confluence level (in which fewer cells were replicating), the damage levels on fibronectin remained high i.e. there was damage-replication uncoupling on that matrix substrate.

Cells are favorably settled in the micropattern and even at higher confluence levels, when the cells start to overgrow the ridges of the micropattern, these cells align themselves in the direction of the micropattern.

The growth curves of EPT1 and EP156T cells clearly displayed different behavior when cells were grown at high confluence.

In order to transform EP156T cells to prostate cancer cells, the cells were kept growing at passage 43 at high confluence to select cells that survived contact inhibition.

At the start of each differentiation procedure at high confluence of the cells, phosphate buffered saline (PBS) (Gibco) was used to wash the cell layer once.

Yet further prolonged treatment with RA and FGF4 still increases PDX1-expression, but at this point cells could start to deteriorate, probably due to high confluence.

When we examined cell cycle progression by flow cytometry, we observed a blockade at G0/G1 phase in MEFs reconstituted with Stat1 WT when cells were maintained at either low or high confluence (Fig. 4C).

Flow cytometry analysis revealed a decrease in the proliferation of the cells described above after 2 and 3 days in culture, in which cells reached high confluence (data not shown), and thus exhibited reduced activity of Pol I (Figure 3C, lane 4 versus 5).

Although Cyclin E-Cdk2 activity declined with the increased density of all MEFs, the ability of either Stat1 WT or Stat1S727A to further inhibit CyclinE-Cdk2 activity was still evident in cells maintained at high confluence (Fig. 4B, see quantification in right panel).

The observed lower level of total RNA in high confluence cells treated with 10 μg/ml prolactin, and potentially the inhibitory presence of CIS, may explain the reduction in signal observed in higher confluence cells at 10 μg/ml.

The migration/homing ability of MSCs has been known to be affected by passage number and high confluence [ 37– 37].