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Whereas C1INH showed no significant binding even at the highest concentration (data not shown), specific protein interactions with CS-A were found for C1q, C4BP and factor H, thereby confirming the affinity chromatography results.
However, slight changes in morphology were observed for Vero cells at the highest concentration (data not shown).
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Low and high concentration data were merged for data analysis.
Where Guinier plots revealed aggregation due to high concentration, data were removed (Guinier and Fournet, 1955).
(1) For identification of OP-specific gene changes, we removed the fenamiphos low concentration and the dichlorvos high concentration data from consideration.
In the SGSM 49-treated cells, we did observe a slight decrease in the level of NICD generation at a concentration of 30 μM; however, at this extremely high concentration, we also observed a decrease in holo-APP and β-actin levels that was supported by toxicity measurements at this very high concentration (data not shown).
In all cases, the lifetime MoE (difference between human exposure and the TD05) is greater than 5,000 (i.e., cancer risk level is less than 1 in 100,000), even without adjusting the higher concentration data reported downward to the EC ML.
Pre-treatment of SOAE even for 2 hrs significantly inhibited the inflammatory gene expressions in a dose-dependent manner (Supplementary Fig. 2A), whereas a slight but insignificant increase was observed in TNFα at higher concentrations (data not shown).
Experience with PSOA indicates that its stability in water is influenced by its aqueous concentration, with low ppm levels of PSOA showing less stability than higher concentrations (data not shown).
The wild type JH2-2 strain was not affected in its growth at 20 mg/ml of lysozyme and even at higher concentrations (data not shown).
Gemcitabine and the TYMS-specific raltitrexed showed EC50 values on the nM scale (Figure 6) against the GBM cell lines which all expressed significant levels of TYMS, whereas 5FU was only effective at 1000-fold higher concentrations (data not shown).
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