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Many studies indicate that most of the active plant extracts show their highest activity against Gram-positive strains such as S. aureus[ 30, 31].
We determined that daptomycin had the highest activity against B. burgdorferi persisters among all the active hits (Table 1, Figure 3D) but had a high MIC (12.5 25 µg/mL) against log-phase organisms (Table 2).
Moreover, fluorescence-activated cell sorting analysis revealed that compound 14 showing the highest activity against HeLa (IC50 = 2.51 μM) displayed a significant effect on G2/M cell-cycle arrest in a concentration-dependent manner in HeLa cell line.
In particular, compound 8f showed the highest activity against HBV infection and HBV-related liver cancer.
Compounds 6j and 6o showed the highest activity against E. coli and S. aureus.
Compounds 4c, 4g and 6j exhibited the highest activity against C. albicans.
The compound 2 had the highest activity against Helicobacter pylori (MIC 3.9 μg/mL).
ITZ, KTZ and POS showed the highest activity against M. pachydermatis strains, whereas MICO TER and FLZ the lowest.
In addition, compound 17 exhibited the highest activity against erythromycin-susceptible S. pneumoniae ATCC49619 and Staphylococcus aureus ATCC25923 as well.
Laboratory investigations showed that the artemisinins display their highest activity against the juvenile stages of the parasite.
Compounds 3g, 3j, and 5c showed the highest activity against methionine synthase (IC50: 20 μM, 18 μM, 9 μM, respectively).
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