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The distinction of major groups followed the commonly accepted higher-level classification of eukaryotes [22] modified according to a phylogenomic study [23].
Based on the trees obtained, we evaluate the current higher-level classification of the Rogadinae.
Over the past two decades, DNA sequence data has brought additional resolution to the higher-level classification.
As mentioned previously, anomurans have undergone dramatic changes in higher-level classification based on recent phylogenetic studies.
Here, we investigate the phylogenetic history of the Rogadinae, revise their higher-level classification and assess the evolution of their host ranges and mummy types.
These studies have dramatically increased our understanding of anomuran relationships and resulted in several major changes within higher-level classification [ 17, 18, 27].
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However, other taxonomists believe that some patterns of characteristics are sufficiently stable to make higher-level classifications worthwhile, although there are different views about what the higher-level classifications should be.
Genetic characteristics and higher-level classifications need to be clearly and unambiguously defined in order to avoid errors and inconsistencies in downstream clinical applications.
Although RDP was marginally better than SILVA and Greengenes, it did not perform well for higher-level classifications, with 9% of the OTUs not classified to a phylum.
The main comparisons of microbial diversity within and among horses were made for genus and higher-level classifications as described below.
Our results challenge current higher-level classifications, reveal the abundance of genetic diversity in the genus that is potentially available for crop improvement, and provide a valuable resource for species delineation, germplasm conservation and use.
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