Sentence examples for higher transposition efficiency from inspiring English sources

We report the identification of the shortest piggyBac TRDs, micro-PB, which have a higher transposition efficiency in HEK 293 than that of the previously reported piggyBac minimal terminal repeat domains, mini- piggyBac.

The Minos transposon, from Drosophila hydei, has attracted interest because it shows a low insertional bias and high transposition efficiency in a range of animals (for a review, see [261]).

All four binding sites are required for transposition and, in general, the closer the IR/DRs, the higher the transposition efficiency [243].

However, it is possible that such an event would be deleterious and that the cell would be eliminated [248] and, as SB transposition efficiency is higher for methylated [253] and heterochromatic [254] transposons, excision of transposons involved in gene disruption may be relatively rare.

Moreover, because transposition efficiency is high and the NGS library preparation protocol is robust, we are able to multiplex several different transposon constructs in the same library.

When transferred as part of the integrative plasmid into M. methylotrophus, these units, like earlier used mini-Mu LR), could be integrated into the bacterial chromini-Mu LRh the formation of a couldegrate; the transposition efficiency of this process was equally high whether the helper was (E-plus) or (E-minus).

Use of a more efficient transposase could also increase transposition efficiency.

Transposition efficiency is directly dependent upon increased transposase levels up to a certain threshold level [37]; therefore, our results may reflect this increased transposition efficiency and a corresponding deleterious effect on viability due to increased integration events with higher probability of disrupting essential genes and/or regulatory elements.

Thus, higher level of sequence variation in mGing family compared with the mPing family might account for the different transposition efficiency between two MITE families.

Conjugation with pHB14 plasmid and induction of the transposition in S. meliloti produced approx. 3.22 × 104 transconjugant colonies harboring the fluorescent marker with the transposition efficiency of 0.8%.

Although Pong transposase, which was shown previously to catalyze higher transposition frequency than Ping, was used in this experiment to facilitate the yeast transposition assays, its catalytic mechanism is likely indistinguishable from Ping transposase25.