Sentence examples for higher transformation efficiency from inspiring English sources

The latter approach yields higher transformation efficiency (4~5 transformants per μg of DNA), but the former has the potential to mass-produce competent cells for future needs.

The gene gun transformation showed higher transformation efficiency than the ones transformed using Agrobacterium when targets were bombarded once or twice using 280 psi of helium pressure at 6 to 8 cm distance.

The floral-drop transformation using the optimal floral-dip conditions showed higher transformation efficiency than the floral-dip method, but the dropped flowers turned brown and died within 2 d.

The transformation efficiencies of Agrobacterium tumefaciens strains AGL1 and EHA105 were compared with the Agrobacterium density at OD600 = 0.8 1.0 and the co-cultivation at 25 °C for 48 h. A. tumefaciens strain EHA105 gave slightly higher transformation efficiency than AGL1, with statistically non-significant difference.

In this research, optimum weight ratio of 1 10 of plasmid DNA to copolymer P-123 was screened to achieve higher transformation efficiency.

We hypothesized that the smaller size of the T-DNA sequence harbored by pABr3, corresponding to approximately 1/3 of the sequence contained in pABr1, may result in a higher transformation efficiency, thus increasing the number of transferred T-DNA copies.

Use of DNA methylation-impaired E. coli donor strains resulted in 100-fold higher transformation efficiencies, indicating that DNA methylation recognition systems are operable in the organism.

Some previous studies reported that higher transformation efficiencies were achieved with DNAs isolated by electroelution than with β-agarase [ 24, 26].

In conclusion, with the optimized approach, a high transformation efficiency of 145 transformants per 10 conidia was obtained.

Both genes were successfully transformed into Y. lipolytica Po1g with a high transformation efficiency, about 3,000/μg DNA.> -wrap-foot> The strain used for the enzyme expression was Y. lipolytica Po1g (MATa ,leu2-270 ,leu2-270 ,ura3-302 ,ura3-302 URA3 ,xpr2-332 ,xpr2-33227].

In addition, yeasts are attractive hosts for the high throughput screening of peptides or proteins from large combinatorial libraries (Chao et al. 2006; Li et al. 2007; Ueda 2004), with a high transformation efficiency of plasmids of up to 1 1.5 × 108 transformants/μg achieved (Benatuil et al. 2010).