Exact(36)
The latter approach yields higher transformation efficiency (4~5 transformants per μg of DNA), but the former has the potential to mass-produce competent cells for future needs.
The gene gun transformation showed higher transformation efficiency than the ones transformed using Agrobacterium when targets were bombarded once or twice using 280 psi of helium pressure at 6 to 8 cm distance.
In general, higher transformation frequencies were observed in E. coli O157 H7 9×1022 to 1.25×104 transformants/µg) than in Salmonella (1.2×102 to 1.4×103 transformants/µg).
Transformation with constructs carrying sequences homologous to the genome typically results in 10-fold higher transformation frequencies then the use of non-homologous constructs, and among these transformants a high proportion shows integration of the construct at the homologous genomic locus [ 3, 12].
In our cohort, 37 (3.72%) of 994 cases of oral submucous fibrosis transformed into malignancies with an average duration of 37.42 months, which was a higher transformation rate (1.9%) and a shorter mean duration for transformation (52.3 months) than in our 1991 2001 study [ 7].
The Orelia soil showed significantly (p < 0.001) higher transformation than the Immokalee soil.
Similar(24)
In conclusion, with the optimized approach, a high transformation efficiency of 145 transformants per 10 conidia was obtained.
A maximum number of transformants with a minimal amount of DNA requires a high transformation efficiency.
High transformation efficiency and accuracy were achieved as ~63% of the transformants was confirmed to be correct.
In general, electroporation offers the highest transformation efficiency, and is especially useful when small quantities of DNA are transformed.
The highest transformation rate was found in migraine and probable migraine (PM) groups.
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