Sentence examples for higher repression from inspiring English sources
Although this observation confirmed that a decreased CodY response, that is a higher repression of transcription of CodY-regulated genes, improves protein overexpression, this strategy would compromise the chemically-defined nature of GCDM.
Alternatively, rapid early platelet decline may represent higher repression of megakaryopoiesis, or increased platelet consumption.
siFLuc-II also showed significantly higher repression at the coding region of the FLuc segment.
Conversely, the two siRLuc showed higher repression when the RLuc segment was in the 3′-UTR.
This study elucidates whether the coding region or 3′-UTR elicits higher repression.
Results showed that the two siFLuc elicited higher repression when the FLuc segment resided in the coding region.
All the tested gRNAs in this strain repressed gfp expression to some extent, with highest repression observed with c6 and c7.
poxa1b showed the highest repression level of all genes evaluated, in both fermentations conducted with dyes added and seems to be the most affected by copper and/or dye among the P. otreatus laccase transcripts analyzed in this research, with this response possibly being growth time dependent.
Specifically, for any given time point, the prediction target with the highest induction has a rank 1 and the gene with the highest repression has a rank 50.
It is recommended that the 50 genes should be ranked according to their relative induction or repression relative to the expression levels observed in the wild-type parental strain in the absence of 3AT, such that the gene with the highest induction has a rank 1 and the gene with the highest repression has a rank 50.
Western blot analyses of TetR Caco2-BBE cells transfected with three CD98-shRNA constructs after 2, 4 or 6 days of treatment with 2.5 or 5 µg/ml tetracycline revealed that only the CD98-shRNA3 construct (see Materials and Methods) was effective in knocking down CD98, with highest repression effect after 2 days of 2.5 µg/ml tetracycline treatment (Fig. 5C, left panel).
