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Cy0 method resulted in higher quantification values, leading to the decrease of total variability in CASP3 and LYZ genes.
The anorexic bacterial profile is similar to the lean control group for the Firmicutes, Bacteroidetes, and Lactobacillus but different for M. smithii (anorexic group had higher quantification).
We have previously compared both methods obtaining higher quantification in wastewater samples when applying the first one [ 37].
The leg and lung tissues of 1 rat and the leg tissue of another rat tested positive, albeit at considerably lower quantities (higher quantification cycles) than pla values measured in peritoneal samples.
The viability of C. botulinum was confirmed for all three sample types (slug carcasses, slug feces and live slugs) by visible growth in enrichment media combined with obtaining a higher quantification cycle (Cq) value than from the non-enriched samples.
(A – A″ ) Dia protein levels are similar (∼two fold higher, quantification not shown) in tracheal cells expressing the GFP-Dia-FL construct and in the adjacent posterior spiracle cells (S).
Similar(52)
Combustion IRMS and ferrographic enumeration had the lowest quantification limits (∼2 to 20 cells/ml), whereas microplate spectrofluorometry had the highest quantification limit (∼105 cells/ml).
In contrast, the autumn season was more variable (F = 140.377; df = 9; P = 0.000) with three significantly differentiated groups: Gaia presented the highest quantification level, Boadella Reservoir in an intermediate position and La Baells, La LLosa and Sant Ponç Reservoirs with the lowest and similar quantification levels (Fig. 1).
Results from thirteen mobile phase compositions showed that the mobile phase composition of ACN/NaAcetate (5 mM /MeOH/Acetic acid (pH 3); (30:40:30; v/v/v) resulted in the highest quantification value for OTA.
The highest quantification limit was found for hexanone which was the least abundant compound in chromatograms acquired during the calibration procedure.
All three DEHP metabolites as well as the PFAAs had relatively high quantification rates.
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