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To achieve this, we used second generation sequencing exclusively, including ROCHE-454 GS FLX Titanium sequencing for its read length advantage and Illumina GA2 sequencing for its higher quality reads.
367 additional ESTs were used but not deposited in DDBJ because they were superseded by higher quality reads for the same clone.
Preliminary sequence analysis revealed that 5' end sequencing of the EST's provided higher quality reads than those generated from the 3' end.
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After filtering, 70,453,120 high quality reads were obtained and used for de novo assembly (Table 1).
As additional quality criteria we used only variants with a coverage >10× of high quality reads.
The remaining high quality reads were checked for the existence of chimeric sequences.
The GS20 has standard software to recognize high quality reads and convert the signal (light) into a base call.
They were regarded as high quality reads.
This resulted in 1,517,878 high quality reads.
Thus filtered, high quality reads were used for further analysis.
For this purpose, all 1,063,520 high quality reads were used.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com