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This procedure assumes that after filtering, a higher mean ConQuass score is achieved because the remaining proteins have a higher quality alignment.
Split reads may be identified from single-end or paired-end sequencing, although using paired-end data has the advantage of higher quality alignment.
We used different alignment algorithms for these 2 data sets because in each case one program gave a higher quality alignment than the other.
In addition, eukaryotic and Prosthecobacterial tubulins were realigned without artubulins and FtsZ, in order to obtain an extended, higher quality alignment, and a tree was constructed using TreeFinder (Additional File 4 ).
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The HD DBDs were aligned using MAFFT (Katoh et al., 2005), which gave higher quality alignments, with fewer gaps, than other programs.
Both programs ran with default settings and took advantage of the ABI SOLiD colour-space format for higher quality alignments.
Alignments were processed in order of increasing E-value, to prevent lower quality alignments from disrupting formation of families using higher quality alignments.
At the least, paired-end reads yield higher quality alignments, because of the extra constraints on mate pair distance and alignment orientation.
An exon was considered to show allele assignment error when reads originating from one parent were assigned (based on higher quality alignments to the wrong reference) to the other parent more than 5% of the time.
The purpose of this study was to create and validate an aligner with the goal of quickly generating a high quality alignment and having the flexibility to use any reference alignment.
This strategy allowed us to make a high quality alignment for the first ∼1075 aa, and the last ∼500 aa.
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CEO of Professional Science Editing for Scientists @ prosciediting.com