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High purity grade BTB was purchased from Daejung Chem.
All chemicals and reagents were of high purity grade.
Dimethyl sulfoxide (DMSO) with high purity grade of 99.9% was acquired from Sigma-Aldrich.
All reagents used were available commercially and were of high purity grade.
High purity grade type I collagen was extracted and purified from bovine pericardium.
Methanol (HPLC-grade), ammonium acetate, disodium hydrogen phosphate, and formic acid of the highest purity grade were purchased locally.
All other chemicals were from Merck Ltd., SRL Pvt., Ltd., and were of the highest purity grade available.
All other chemicals were from Merck Ltd., SRL Pvt., Ltd., Mumbai, and were of the highest purity grade available.
Simultaneously, residual monomers were removed from the polymer product with the escaping CO2-gas, achieving a high purity grade.
Ultra high purity grade nitrogen was bubbled through the mixture to evaporate the ethanol under fume hood, thus depositing thiol –SH on the surface of the silica.
High purity grade RhB was purchased from Daejung Chemical & metals Co.. RhB aqueous solution 300 ml with the concentration of 1.3 × 10-2 mM was prepared.
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