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These beneficial effects were most obvious at higher passage numbers.
However, similar blastocyst rates were achieved when using higher passage (numbers 9 12) EG cells as donor cells.
In conclusion, the present study suggests that the epigenetic status of EG cells change with increasing passage numbers, and that higher passage number EG cells are better primed for SCNT.
Invasive properties of immortalized MEFs did not change with higher passage number.
However, when these cells were serially passaged in culture to higher passage levels (p256), they were found to be tumorigenic when injected into newborn nude mice [11].
Many colonies appeared fragmented at early passages and at higher passage numbers, compact colonies disappeared and the colonies broke up into single cells.
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Importantly, gingival MSCs display stable morphology and do not loose MSC characteristic at higher passages.
An increase in nestin mRNA expression was observed in cells as they reached higher passages.
These findings are in line with the observed enlargement of the membrane compartment and vacuole formation in higher passages.
Furthermore, our results indicate that adipogenic differentiation potential decreases upon replicative senescence whereas the propensity for osteogenic differentiation increased in higher passages.
1257 ESTs (including 721 non-redundant genes) were significantly up-regulated in higher passages, whereas 698 (including 481 non-redundant genes) were down-regulated (figure S5; table S2).
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