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However, these higher order forms of ectodomain were unstable on mild reduction with DTT.
We expressed untagged and tagged TSHR ectodomains (residues 1 412) in mammalian cells and found higher order forms of the receptor under non-reduced conditions.
These animals, however, have very limited cognitive functions in comparison to terrestrial mollusks, which are capable of higher order forms of learning and memory [8], [9].
Distinct bands of 250KD corresponding to higher order forms of the ectodomain was observed in untreated and TSH treated samples under non-reduced conditions (Figure 2A).
Therefore, to understand the exact biological role of dimers and/or higher order forms of receptors it will be necessary to generate GPCRs that are expressed only as monomeric or multimeric receptor forms in model cell systems.
These observations suggest that it may take both monomeric and multimeric forms of receptor to coexist for the normal physiological functions of thyrocytes as suggested for other GPCRs [23].Therefore the role of higher order forms of the TSHR in physiology and pathology of the thyroid remains open to investigation.
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Piper analyzes "higher-order" forms of discrimination in which certain traits, such as speaking style, come to be arbitrarily disvalued on account of their association with a disvalued race or sex (2001).
Hence, the data demonstrated that TSHR ectodomains were capable of forming higher order forms in the absence of the transmembrane domain.
The blots when probed with a TSHR antibody M4 (RSR 4: recognizing residue 322 341) [27], revealed the presence of higher order forms in addition to the fully mature monomeric form of GPI: TSHR Ecd consisting of a doublet comprising of a 100KD form and less mature molecular species of 74KD as observed by others [28].
As an example, we prove a closed high-order form of the classical Jacobiʼs formula.
An SDS-resistant higher-order form of TssK with an apparent mass of ~100 kDa was also observed on analysis by SDS/PAGE.
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