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The higher melting peak is related to the orientation of molecular chains.
However, the value of the higher melting peak Tm2 stayed almost constant with Tc.
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This unusual behavior was attributed to the flow induced crystallization in extended chain formation at the skin and ease of inclusion of HV defects in the HB crystals that formed at slower cooling conditions in the interior creating thinner HV rich crystals with lower melting and thicker HV poor crystals with higher melting peaks.
The melting curves showed two melting endotherms, a higher melting (HM) peak at a constant temperature of 105°C and a lower melting (LM) peak at temperatures depending on the crystallization temperature Tc.
Ιt is long known [ 1] that the β-phase if cooled below 100 110 °C, as in the present case, is susceptible to βα recrystallization followed by melting of the thus formed α-form, which contributes to the high-temperature melting peak.
The possible reason for the absence of melting peak could be due to the impregnation or encapsulation of the drug within the viscous polymer matrix and PU with its high melting point has restricted the contact of the melting drug with the base of the aluminium pan thus leaving no signs of its melting to the detector.
High quality melting peaks were obtained in 136, 154 and 171 assays, enabling accurate determination of sample genotypes.
Analyses employing unpurified blood samples were extremely efficient yielding high quality melting peaks with the majority of samples, demonstrating first time pass rates between855% and 95% (mean = 91.2%).
As the received dose was increased, however, the high melting temperature peak was gradually diminished and finally disappeared after 1.8 MGy e-beam radiation, suggesting that the double melting phenomenon was mainly attributed to the melting/re-crystallization/re-melting behavior.
SYBRGreen assays on NK603 with method 5 also showed an additional product with a higher melting temperature than the peak of the expected product.
Moreover, a relatively high end-point fluorescence signal should directly result in a relatively high DNA melt peak during post-PCR melt curve analysis (vide infra).
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