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As shown in Figure 1, higher lipase activity was obtained when glucose was used as primary carbon source.
The results suggested that the increased secretion of the chimeric lipase was the main factor for the much higher lipase activity of the chimera in the supernatant but the improved catalytic rate of the chimera only contributed a little.
Highly aggressive, estrogen receptor negative (ER-ve) MDA-MB-231 cells possess higher levels of PLD1 protein, and therefore, these cells have 8-fold higher lipase activity when compared to low invasive MCF-7 cells, which have relatively low levels of PLD1.
To test whether higher lipase activity was indeed instrumental in generating higher TAG deposits in itpr ku, inhibition of mid-gut lipases was attempted by introducing a lipase inhibitor, Orlistat (Sieber and Thummel, 2009) in the diet of CS and itpr ku adults.
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Orally administered DEHP undergoes presystemic hydrolysis catalyzed by lipase to MEHP in the gut, with mice exhibiting higher lipase activities in the small intestine compared with rats and marmosets (Ito et al. 2005; Kessler et al. 2004; Pollack et al. 1985).
Bacterial isolates often reveal high lipase activity (Martinez et al., 1996), although only 41 of 115 sequenced bacterial genomes contained identified lipid transporters.
Based on the analysis of ridge max, the optimum immobilization conditions were as follows: immobilization time 2.3 h, immobilization temperature 13.3 °C, and enzyme/support ratio 0.41 (w/w); the highest lipase activity obtained was 1196 U/mg-protein.
The optimal medium consisted of 2.27 g/L yeast extract, 2.04 g/L tryptone, and 0.29 g/L glutamate, in which the highest lipase activity for the substrate, p-nitrophenol butyrate, and the protein level were 2160 ± 191 U/L and 410 mg/L, respectively.
Liver extract showed the highest lipase activity compared to lipase from P. cepacia.
The highest lipase activity was retained at 55% when incubated in 1.5 and 2% (v/v) of SDS.
The highest lipase activity (140 U/mL) as observed in the cultivation with acid pre-treated coconuts dregs without the supplementation of coconut oil showed that B. stratosphericus prefers the readily available oil in the coconut dregs rather than the supplementary oil as an inducer for lipase production.
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