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Our original assumption was that higher levels of differential gene expression would correlate with higher levels of alternative splicing.
Such a fixation of alleles could result in higher levels of differential expression.
As an alternative to DAVID, we also used LRpath (17) to identify functional groups with significantly higher levels of differential expression.
In comparison to the differential gene expression achieved by exposure to NaCl and glycerol, cells exposed to KCl showed overall higher levels of differential gene expression.
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A lower correlation coefficient indicates a higher level of differential regulation of the Bead-array 22,418 test-genes in the spinal cord samples under investigation.
For all isoforms from significantly differentially spliced TSS, the more stringent rule of absolute value of dIF above 20 was introduced in order to assess the isoforms with the highest levels of differential splicing.
Ch3L1 showed the highest levels of differential expression in TM versus SC cells.
This interpretation is consistent with the relatively high levels of differential gene expression observed for samples in these three clusters.
Formerly available methods for expression profiling usually had to apply filtering criteria like signal intensity, "Present" calls and stringent cutoffs for high levels of differential expression to avoid false-positive calls.
For G12, all four candidates demonstrated high levels of differential expression.
In contrast, our PSRP 91 gene TaqMan assay did not require genes to have high levels of differential.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com