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As expected, the pathway-level differentiated genes did not display an obvious gene-level consistency in their relative expression levels across the sample population even in the same class (Figure S1), although there may be higher levels expression in general in the Inclusion class (NGT) compared to Exclusion class (IGT+DM2).
Furthermore, the levels of LUC expression seen in hsi2-4 planthathat carry these reporter complexes correlate with transgene copy number, with relatively low levels of LUC expression seen in hsi2-4, Kan S plants that contain two GSTF8 LUC copies and correspondingly higher levels expression in hsi2-4, Kan R or hsi2-4 linesines with five and seven total reporter gene copies, respectively.
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We therefore over-expressed active Caspase-9 (high level expression) in combination with the inactive proforms of Caspase-3 or -7 (expressed at low levels) (Figures 2G, 2H, S5).
Plasmids expressing FPs from Psmyc gave high-level expression in M. smegmatis, M. marinum and M tuberculosis.
It was also codon-optimized for high-level expression in Escherichia coli k12.
However, sialin shows widespread and high-level expression in adult tissues.
A synthetic gene encoding CtxB was designed for high level expression in plant cells and cloned as ubiquitin (Ub) fusion in a plant expression vector.
To this end, a synthetic gene, encoding a mature wheat nsLTP1 polypeptide, was designed to ensure high level expression in Escherichia coli.
Most such promoters either lack high-level expression in certain muscle types or are too large for inclusion in rAAV vectors encoding microdystrophin.
A synthetic gene coding for the surface glycoprotein (G protein) of rabies virus was strategically designed to achieve high-level expression in transgenic plants.
Efforts aimed at recombinant expression of functional striated muscle myosin isoforms in bacterial or insect cell culture have largely met with failure, although high level expression in muscle cell culture has recently been achieved at significant expense.
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