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On day 3, the cells showed a significantly higher level of viability on the GAL-PDMS than on the pristine and collagen-coated PDMS.
The islets exhibited a higher level of viability and functionality compared to the free islets control [ 116].
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The high level of viability of the examined cells in contact with the nanotubes, the slight increase of collagen formation, the lack of pro-inflammatory IL-6 cytokine as well as the induction of free radicals, confirm a good biocompatibility of nanotubes, which is similar to that of polysulfone currently used in medicine.
Less than 5% of these cells stained with trypan blue, indicating a high level of viability.
The cells were observed to have populated the scaffold with a high cell density and a high level of viability.
These results confirmed that a high level of viability of the microalga is maintained, even at the highest concentration of chromium tested.
However, as mentioned above, unlike M. bovis BCG, M. tuberculosis retained a relatively high level of viability after 2 months of incubation in stationary phase.
The high level of viability and the uniformity of results may be attributed to the fact that spores were produced in vitro, under uniform environmental conditions and with no growth constraints.
First, we tested the cytotoxicity of increasing concentrations of chaetocin in the cells for all three PD levels, and found that cell cultures of all senescence states maintained a high level of viability when treated with 5 or 10 nM chaetocin and decreased rapidly at higher concentrations (Fig. 7A).
The cells exhibit high levels of viability, proliferation, three- dimensional (3D) cell morphology and other functions in the constructs.
High levels of viability can be achieved by minimizing the volume of the solution used for vitrification of mammalian embryos [ 31].
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