Those targets with the greatest PITA score demonstrated a high level of repression, in the order of 28 42% expression relative to our controls.
This gene displayed the highest level of repression, by a factor of 5 at an acetoin concentration of 200 mM and a factor of 10 at an acetoin concentration of 300 mM.
Even in reports describing the function of the uORF itself, the role of the translated peptide is primarily a cis-acting one, either causing ribosome stalling at the termination codon of the uORF and a high level of repression of the main CDS [ 25, 26] or, more occasionally, affecting mRNA stability.
During the Spanish Civil War (1936 1939), the Canary Islands suffered one of the highest levels of repression by the insurgent side, even though there were no battles in the islands.
This CRISPR based interference (CRISPRi) not only showed high levels of repression but also no evidence of off-target effects by RNA-seq.
Gene expression decreased 2- to 36-fold under shading conditions, with high levels of repression of the expression of anthocyanin-specific branch genes DFR, ANS, 3GT, 3MT1 and 3MT2.
Notably, the highest level of transcriptional repression was detected in Dusp6 (d-value = -16.4; R-fold = 0.09), a dual-specificity phosphatase acting in Ras signaling pathways.
It was also evident that SACMV was able to maintain a high level of transcript repression as virus infection persisted (67 dpi), and because cassava is a vegetatively propagated crop, systemic infection can persist for months until harvest.
However, for both genes, the species having higher DNA methylation levels also showed higher gene expression levels, which is inconsistent with the expected repression of gene expression by a higher level of DNA methylation in the promoter.
On the contrary we see a high level of de-repression in cells grown on limiting glucose (Table 6).
