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In addition, the higher level accumulation of heme can lead to inhibition of the synthesis of Chls in plants by feedback regulation (Guo et al. 1998; Terry and Kendrick 1999; Brumann et al. 2014).
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Inactivating calM by PCR targeting, caused high level accumulation of N-demethyl calcimycin.
Importantly, the MR imaging studies showed notable signal enhancement (44.9 ± 4.2%) on the tumor, indicating a high level accumulation of the contrast agent within the PC-3 tumor sites.
JNK2 silencing results in high level accumulation of non-phosphorylated c-Jun in HCT116 cancer cells (see above).
Besides, this study also mentions high level accumulation of a developmental protein namely ripening related protein (RLP) in incompatible interaction after infection (Additional file 5).
The expression profile seems compatible with the high level accumulation of free IAA observed in Arabidopsis pollen [ 65] and mature rice anthers [ 31].
For CMVinII variants this is then followed by induction using estradiol, which resulted in high level accumulation of the proteins tested here (CP, GFP, E1).
Collectively, they demonstrated that very high level accumulation of RPs (up to 5 mg per g leaf fresh weight (LFW)) can be achieved within 7 10 days after infiltration (dpi).
Interestingly, another miRNA166 sequence, gma-miR166a-5p (#11), mapping at the 5' end of the precursor RNA stem loop, accumulated at very low levels in all the tissues, in contrast with the high level accumulation of the gma-miR166a-3p gma-miR166a-3p gma-miR166a-3pe same RNA precursor (#10 in Table 2).
Subsequent expression of the resulting IT construct [scFvC6.5-syncyt2Aa1 mychis6)]2 led to high-level accumulation of the recombinant protein in yeast membranes.
Transgene expression from the chloroplast (plastid) genome offers several attractions to plant biotechnologists, including high-level accumulation of foreign proteins, transgene stacking in operons and a lack of epigenetic interference with the stability of transgene expression.
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