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It was optimal for most samples that had relatively higher fluorescence yield.
Polyphasic chlorophyll a fluorescence transients from the flag leaves of UV excluded wheat plants gave a higher fluorescence yield.
ANS binding to PrP fibrils resulted in higher fluorescence yield and a blue shift in the fluorescence spectrum, as expected for PrP fibril structures, compared to the weak fluorescence yield observed after ANS binding to soluble PrP [ 51].
This second class of edelfosine analogs, Et-BDP-ET and Yn-BDP-ET, containing a BODIPY fluorophore attached to the alkyl chain of edelfosine, had a higher fluorescence yield and resistance to photodegradation than the above PTE-ET and PTRI-ET (Supplementary Information).
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However, these same compounds with customized filters or further derivatives with higher fluorescence yields could be considered as probes for the specific staining of trypanosomes using existing LED-based fluorescence microscopes.
These properties combined with high fluorescence yield and wavelength tunability [96, 124, 125] render lead halide perovskites as ideal materials for lasing.
(B) Melting curves at gain 6 show optimal melting transition for samples with low fluorescence yield while samples with high fluorescence yield showed saturation of fluorescence intensity, and hence, their ΔTm could not be calculated at this gain setting.
GFPmut3.1 variant can be detected after a short lag-phase of only 10 min, it shows a high fluorescence yield in relation to the amount of reporter protein, a good signal to noise ratio and a low detection limit.
In our case of small-molecule compound screening, a high gain setting caused saturation of fluorescence signal resulting in loss of data for high fluorescence yield samples while a low level of gain does not provide sufficient signal to observe the melting transition for low fluorescence yield samples (Figure 2) to accurately measure the protein stability.
High fluorescence yield and good photostability were also sought for, whenever possible.
These compounds exhibit good thermal stabilities and high fluorescence yields.
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