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However, embryos exposed to the temperature step within 20 minutes after fertilization have a much higher fluorescence background (Figure 4E) in comparison to embryos that developed at uniform temperature (Figure S9), suggesting that Bcd not trapped within nuclei or the energid was at least partially mixed, and that the mixing observed was not reversible.
Unfortunately, the results were less satisfactory and the nitrocellulose membranes showed even a higher fluorescence background.
The dense protein network within the GBM results in a higher fluorescence background and significant light scattering.
However, since nylon membranes are known to exhibit a higher fluorescence background compared to nitrocellulose membranes, the latter were also tested.
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Although intracellular staining of these low-abundance molecules is sometimes difficult, especially in samples with a high fluorescence background like tumors, techniques such as fluorescent multilayer or enzymatic amplification staining can be very useful if carefully optimized (90, 91).
Chloroplasts are recognized by their high fluorescence background.
However, their applications are restricted by the high fluorescence background interference of conventional laminating pouches.
In the initial analysis using the diamond holder, the particle RB-QD04-0001 showed neither G- nor D-band but only a high fluorescence background.
We could not obtain good Raman data on this section due to high to extremely high fluorescence background most likely from the surface coating and contamination by the epoxy enclosing the particle.
In a conventional fluorescence microscope single molecule imaging at such a depth within the specimen is almost impossible, because the SNR is low due to a high fluorescence background (see Figure S2).
However, this dye produces a high fluorescence background even without cells.
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