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The labeling is continuous across the modified surface with spots of higher fluorescence (Figure 4e,f).
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Thus, fungal cells contain higher amounts of DNA resulting in high fluorescence (Figure 1).
Using vasa::egfp transgenic zebrafish as donors, a testicular cell suspension was obtained and subsequently submitted to FACS, in order to enrich transplantable SSC (Figure 4J,K) by sorting for big cells carrying high fluorescence (Figure 4L,M).
These germ cells exhibited high intensity of Zn fluorescence (Figure 3A).
Clearly, the fluorescence signal in Figure 3(c) is considerably less intense than that in Figure 3(a), with the leftmost blastomere in Figure 3(c) exhibiting slightly higher fluorescence intensity.
All samples used were of high quality and integrity, as determined by mRNA fluorescence figure with typical shape of broad peak and without two ribosomal RNA contamination peaks.
Figure 7 shows that CCRF-CEM gets higher fluorescence intensity than the other control groups.
Similarly, GFP diluted in ʟ-arginine exhibited a higher fluorescence intensity compared to GFP diluted in NaHCO3 (Additional file 5: Figure S5).
Ofloxacin-treated rabbit sera showed higher fluorescence intensities between 390 and 540 nm than control sera, with a maximum at 460 nm (Figure 1).
Of particular notice, samples having initial higher fluorescence in baseline cycle, as shown in replicate D3 and replicate D5 from the same sample in figure 1a, are inclined to have higher fluorescence reading in the ensuing cycles.
The results in Figure 2 show that only SOSG fluorescence differed according to the genotype, with significantly higher fluorescence in mutant leaves; there was no significant difference in the DCF and ProxF signals.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com