Briefly, concentrations of phthalate metabolites were quantified using solid phase extraction-high performance liquid chromatography-isotope dilution-tandem mass spectrometry and expressed as wet weights (ng/mL).
Measurements of 11 phthalate metabolites concentrations (see Additional file 1: Table S1) were conducted using isotope dilution on-line solid-phase extraction-high performance liquid chromatography-electrospray ionization-isotope dilution tandem mass spectrometry [ 26].
Using online solid phase extraction high performance liquid chromatography tandem mass spectrometry, we measured nine ATZ-related metabolites in urine that included dealkylated, hydroxylated, and mercapturic acid metabolites.
In addition, samples were taken for BelCaP when administered together at 6 h 15 min, 6 h 30 min, 7, 8, 9 and 24 h after belinostat on day 3. Belinostat and paclitaxel levels were determined in lithium-heparinised plasma using validated solid-phase extraction high performance liquid chromatograpgy (HPLC -tandem mass spectrometric detection (MS/MS) metHPLC -tandeme, Indianapolis, IN, USA).
The Soxhlet technique provided higher performance of extraction and greater amounts of compounds extracted compared to ASE, but similar concentration of estragole.
For example, we can use an existing high-performance extraction algorithm in the first phase and then pipeline the results to our high-precision rule-based algorithm for the second screening.
The preferred method of analysis post-extraction was high performance liquid chromatography tandem mass spectrometry (LC-MS/MS), following the protocols of Mekebri et al. [45].
Everolimus was measured in EDTA-whole blood by an integrated online solid-phase extraction high-performance liquid chromatography single-quad mass spectrometry (SPE-HPLC-MS) system (Kirchner et al, 2001).
Perfluorooctanoate (PFOA), perfluorooctanesulfonate (PFOS), perfluorohexanoate, perfluorohexanesulfonate (PFHxS), perfluoropentanoate, and perfluorobutanesulfonate (PFBS) in blood plasma and PFOA/PFOS in drinking water samples were measured by solid-phase extraction, high-performance liquid chromatrography, and tandem mass spectrometry detection.
We measured the total concentrations of methyl, ethyl, n-propyl, butyl (n- and iso-), and benzyl parabens with a modification of a method for measuring other environmental phenols in urine (Ye et al. 2005a) using online solid-phase extraction high-performance liquid chromatography tandem mass spectrometry (SPE-HPLC-MS/MS) (Ye et al. 2006).
