Pre-treatment of SOAE even for 2 hrs significantly inhibited the inflammatory gene expressions in a dose-dependent manner (Supplementary Fig. 2A), whereas a slight but insignificant increase was observed in TNFα at higher concentrations (data not shown).
Experience with PSOA indicates that its stability in water is influenced by its aqueous concentration, with low ppm levels of PSOA showing less stability than higher concentrations (data not shown).
The wild type JH2-2 strain was not affected in its growth at 20 mg/ml of lysozyme and even at higher concentrations (data not shown).
Gemcitabine and the TYMS-specific raltitrexed showed EC50 values on the nM scale (Figure 6) against the GBM cell lines which all expressed significant levels of TYMS, whereas 5FU was only effective at 1000-fold higher concentrations (data not shown).
Similar peak shifts are observed in all titration series at constant EGFP at higher concentrations (data not shown).
This is unlikely due to the surfactant activity of the conjugate as PEG5K-SS-FTS2 showed minimal hemolytic activity at even much higher concentrations (data not shown).
At lower concentrations, when incubated in the dark, the radicles of the seedlings were elongated, while the photoactivated growth inhibition seemed to happen at higher concentrations (data not shown).
The OE19 cell line is also apoptosis-incompetent in response to cisplatin and 5-FU – and will undergo MC in response to curcumin – although at much higher concentrations (data not shown).
