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A much higher catalytic efficiency could be achieved by substrate-feeding operation.
A lower Km value indicates a higher catalytic efficiency of the enzyme towards its substrate.
Consequently, the G126S and R133L mutants show higher catalytic efficiency as compared with the wild type.
The obtained W361F exhibited higher activity, higher catalytic efficiency, and predicted lower binding energy than the wild-type enzyme.
Then a mutant S63M with a 4.5-fold higher catalytic efficiency than Lip-T was obtained by site-saturation mutagenesis.
The two most active mutants showed up to 70-fold higher catalytic efficiency than the parental GST A2-2.
The amount of endogenous PQQ is generally regarded as a bottleneck to achieving higher catalytic efficiency of PQQ-dependent dehydrogenases.
It could be concluded that coated Cu/Al2O3-honeycomb catalyst showed higher catalytic efficiency than the powdered catalyst in the ozonation process of alachlor.
Therefore, the double mutations T166A/E539K and K312E/E539K were designed in the hope of obtaining mutants with higher catalytic efficiency and better thermostability.
The results of kinetic analysis revealed that rAgaB-4rAgaB-4gher catalytic efficiency (Kcat/Km) toward HMP agarose than that toward LMP agarose.
There is also endo-arabinanase with higher catalytic efficiency, such as Abn2 from B. Subtilis, of which the K m was 2.0 mg/ml [22].
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